The effect of water concentration on the rate and expansion of demineralized dentin matrix

Abstract

The purpose of this study was to test the rate and expansion of air-dried, collapsed, demineralized dentin matrix using three different water-HEMA solutions. Solutions of 10, 25 and 50% water (by weight) were formulated with corresponding amounts of HEMA. Eighteen dentin specimens (0.2 mm thick) were demineralized in 17% EDTA for 5 days, yielding type I collagen disks approximately 200 J.llTI thick. A linear variable differential transformer (L VDT) was used to measure vertical dimensional changes in the demineralized dentin specimens. The specimens were airdried with compressed air, then one of the three test solutions was applied, and the rate and amount of expansion was measured. Repeated measures AN OVA with least squares means comparison test revealed a significant (p < 0.0001) relationship between water concentration and both the rate and amount of re-expansion of dried, demineralized dentin. Hansen's solubility parameter for hydrogen bonding forces was used to express the ability of the different solutions to interact with the demineralized dentin matrix.