Perfluorooctanoic acid reduces viability and gene expression of peroxisome proliferator-activated receptor alpha and estrogen receptor alpha in MCF-7 cells

Abstract

Perfluorooctanoic acid (PFOA) is an endocrine disrupting compound found in food, water, clothes, and other consumer products. It is known to accumulate in the environment and can be taken up through ingestion, inhalation, or skin contact. It has a half-life of nearly four years in humans. PFOA has been shown to bind and activate peroxisome proliferator-activated receptors (PPARs), which are transcription factors found in mammalian cells. PPARs regulate numerous cellular activities, including proliferation and differentiation. Several studies have suggested crosstalk between PPARs and estrogen receptors (ERs). This study aimed to examine the effects of PFOA on cell viability and on PPAR and ER gene expression in MCF-7 breast cancer cells. The results showed a decline in cell viability after 48h of PFOA treatment. In addition, 24h of treatment with PFOA led to a significant decrease in PPARα and ERα, but not PPARβ, PPARγ, or ERβ. Begin Time: 28:30 End Time: 50:40